胶孢炭疽菌生防放线菌gz-8的鉴定及生物活性初步评价
投稿时间:2017-06-27      点此下载全文 HTML
引用本文:张凯,吴曼莉,辜柳霜,郑钧月,柳志强,李晓宇,等.胶孢炭疽菌生防放线菌gz-8的鉴定及生物活性初步评价[J].农药学学报,2017,19(6):708-715.
摘要点击次数: 99
全文下载次数: 
作者单位E-mail
张凯 海南大学 热带农林学院, 海口 570228  
吴曼莉 海南大学 热带农林学院, 海口 570228  
辜柳霜 海南大学 热带农林学院, 海口 570228  
郑钧月 海南大学 热带农林学院, 海口 570228  
柳志强 海南大学 热带农林学院, 海口 570228 liuzhiqiang80@126.com 
李晓宇 海南大学 热带农林学院, 海口 570228  
基金项目:海南省重点研发计划(ZDYF2016155);国家自然科学基金(31560045)
中文摘要:从山东省临沂地区土壤中分离到一株放线菌gz-8,发现其对胶孢炭疽菌Colletotrichum gloeosporioides具有较强的抑制作用,皿内抑菌活性达72.5%。通过形态特征和生理生化测定,结合16S rDNA序列分析,鉴定该菌株为栗褐链霉菌Streptomyces badius。抗菌谱测定结果表明,菌株gz-8对6种植物病原真菌均具有较强的抑菌活性,抗菌谱较广。进一步对菌株gz-8的粗提物进行了生物活性评价,发现其发酵液粗提物可以明显抑制胶孢炭疽菌的菌丝生长(EC50=3.18 μg/mL)和分生孢子萌发(IC50=1.92 μg/mL),且活性明显高于菌体粗提物;同时发酵液粗提物具有良好的热稳定性及pH稳定性;1 000 mg/L的发酵液粗提物对感染胶孢炭疽病芒果果实的防效达到35.94%,与同浓度下百菌清的防效(34.11%)无显著差异。
中文关键词:胶孢炭疽菌  放线菌  栗褐链霉菌  炭疽病  菌株鉴定  生物防治
 
Identification and preliminary evaluation of a biocontrol actinomycete strain gz-8 against Colletotrichum gloeosporioides
Abstract:An actinomycete strain gz-8 was isolated from Linyi, Shandong Province, which had a strong antagonistic effect against Colletotrichum gloeosporioides with the inhibition rate of 72.5%. Strain gz-8 was identified as Streptomyces badius based on the morphology, physiological and biochemical characteristics, combined with 16S rDNA sequence analysis. Strain gz-8 has a broad antifungal spectrum, with strong inhibitory effects on six plant pathogenic fungi. The inhibitory activity of the crude extract from strain gz-8 was evaluated. The fermentation broth extract exhibits obvious inhibitory effects on the mycelial growth (EC50=3.18 μg/mL) and conidial germination (IC50=1.92 μg/mL) of C. gloeosporioides, which had higher activity compared to the mycelium extract of strain gz-8. The fermentation broth extract had good thermostability and pH stability. The control effect of 1 000 mg/L fermentation broth extract to mango fruits infected with C. gloeosporioides was 35.94%, which had no significant difference with that of chlorothalonil at the same concentration(34.11%).
Key words:Colletotrichum gloeosporioides  anthracnose  actinomycete strain  Streptomyces badius  strain identification  biological control
查看全文   查看/发表评论   下载PDF阅读器
&et=4DD8FF9FD26A783823E38A6D538E0DBE3FA7E1EF721DBFA1E7F9E193E1CFB7F3139DD603DEB82EF7DE30C5E82147C9B91E7DC34808FC55518F45A6A3C46CBFAD8E94977B4AFFFDF304920F075667BD949C1775DBDC1B21C9C9FCD85F058211FDE16EBD009D064F5AC7053C984E3CD87402A96CA4D71E9EEA640BA586E0035BF799D5784070DF978B&pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=B6C161C332FA44D9&jid=C858D086464CADF4E2006AC1A8358566&yid=FA004A8A4ED1540B&aid=CF1332E5A7C16DE7882267397677AEE6&vid=&iid=B31275AF3241DB2D&sid=C3BC38F6CC09E835&eid=EB8E83807F36F05B&fileno=20170607&flag=1&is_more=0"> var my_pcid="03F54A49DE00578AA0E5DDF5BC021AA7"; var my_cid="B6C161C332FA44D9"; var my_jid="C858D086464CADF4E2006AC1A8358566"; var my_yid="FA004A8A4ED1540B"; var my_aid="CF1332E5A7C16DE7882267397677AEE6";